Calificación 250cc - Gran Premio de Comunidad Valenciana 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | 1:36.116 |
| 2 | 
Marco Simoncelli |
Metis Gilera | Gilera | +0.334 |
| 3 |
Héctor Barberá |
Pepe World Team | Aprilia | +0.354 |
| 4 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +0.447 |
| 5 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.540 |
| 6 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +0.869 |
| 7 | 
Karel Abraham |
Cardion AB Motoracing | Aprilia | +1.155 |
| 8 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +1.162 |
| 9 |
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | +1.187 |
| 10 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +1.202 |
| 11 |
Raffaele De Rosa |
Scot Racing Team | Honda | +1.249 |
| 12 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +1.461 |
| 13 | 
Jules Cluzel |
Matteoni Racing | Aprilia | +1.633 |
| 14 |
Roberto Locatelli |
Metis Gilera | Gilera | +1.695 |
| 15 |
Mike Di Meglio |
+1.842 | ||
| 16 |
Shoya Tomizawa |
CIP Moto | Honda | +1.969 |
| 17 |
Axel Pons |
Pepe World Team | Aprilia | +2.010 |
| 18 |
Lukas Pesek |
Auto Kelly - CP | Aprilia | +2.175 |
| 20 |
Shuhei Aoyama |
Racing Team Germany | Honda | +2.679 |
| 21 |
Valentin Debise |
CIP Moto | Honda | +3.167 |
| 22 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +4.255 |
| 23 |
Bastien Chesaux |
Racing Team Germany | Honda | +5.489 |
| 24 | 
Imre Toth |
Team Toth Aprilia | Aprilia | +6.302 |
| 25 | 
William Dunlop |
Bigman Racing | Honda | +6.790 |