Calificación 250cc - Gran Premio de Australia 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Raffaele De Rosa |
Scot Racing Team | Honda | 1:33.389 |
| 2 |
Marco Simoncelli |
Metis Gilera | Gilera | +0.225 |
| 3 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.244 |
| 4 | 
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | +0.497 |
| 5 |
Héctor Barberá |
Pepe World Team | Aprilia | +0.539 |
| 6 | 
Mike Di Meglio |
+0.641 | ||
| 7 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +0.725 |
| 8 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +0.803 |
| 9 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +0.896 |
| 10 |
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | +1.199 |
| 11 | 
Karel Abraham |
Cardion AB Motoracing | Aprilia | +1.338 |
| 12 |
Jules Cluzel |
Matteoni Racing | Aprilia | +1.532 |
| 13 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +1.627 |
| 14 |
Lukas Pesek |
Auto Kelly - CP | Aprilia | +1.629 |
| 15 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +1.985 |
| 16 |
Shuhei Aoyama |
Racing Team Germany | Honda | +1.998 |
| 17 |
Roberto Locatelli |
Metis Gilera | Gilera | +2.725 |
| 18 |
Shoya Tomizawa |
CIP Moto | Honda | +3.322 |
| 19 |
Axel Pons |
Pepe World Team | Aprilia | +3.471 |
| 20 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +3.512 |
| 22 |
Valentin Debise |
CIP Moto | Honda | +4.595 |
| 23 | 
Imre Toth |
Team Toth Aprilia | Aprilia | +4.957 |
| 24 |
Bastien Chesaux |
Racing Team Germany | Honda | +5.504 |