Calificación 250cc - Gran Premio de Cataluña 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Héctor Barberá |
Pepe World Team | Aprilia | 1:46.749 |
| 2 |
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | +0.150 |
| 3 | 
Marco Simoncelli |
Metis Gilera | Gilera | +0.159 |
| 4 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +0.332 |
| 5 |
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | +0.376 |
| 6 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.417 |
| 7 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +0.476 |
| 8 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +0.630 |
| 9 | 
Mike Di Meglio |
+0.677 | ||
| 10 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +0.810 |
| 11 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +0.847 |
| 12 | 
Karel Abraham |
Cardion AB Motoracing | Aprilia | +0.976 |
| 13 |
Raffaele De Rosa |
Scot Racing Team | Honda | +0.998 |
| 14 |
Jules Cluzel |
Matteoni Racing | Aprilia | +1.046 |
| 15 |
Shoya Tomizawa |
CIP Moto | Honda | +1.283 |
| 16 |
Roberto Locatelli |
Metis Gilera | Gilera | +1.339 |
| 17 |
Lukas Pesek |
Auto Kelly - CP | Aprilia | +1.352 |
| 18 |
Axel Pons |
Pepe World Team | Aprilia | +3.701 |
| 19 | 
Stevie Bonsey |
Milar - Juegos Lucky | Aprilia | +3.849 |
| 20 |
Valentin Debise |
CIP Moto | Honda | +3.904 |
| 22 |
Bastien Chesaux |
Racing Team Germany | Honda | +4.480 |
| 23 | 
Imre Toth |
Team Toth Aprilia | Aprilia | +4.547 |
| 24 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +5.384 |
| 25 | 
Toby Markham |
C&L Racing | Honda | +6.107 |