Calificación 250cc - Gran Premio de España 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | 1:43.028 |
| 2 |
Héctor Barberá |
Pepe World Team | Aprilia | +0.289 |
| 3 | 
Marco Simoncelli |
Metis Gilera | Gilera | +0.348 |
| 4 |
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | +0.423 |
| 5 | 
Jules Cluzel |
Matteoni Racing | Aprilia | +0.493 |
| 6 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.563 |
| 7 |
Mike Di Meglio |
+0.764 | ||
| 8 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +0.836 |
| 9 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +0.863 |
| 10 | 
Gabor Talmacsi |
Balaton Racing Team | Aprilia | +1.030 |
| 11 | 
Lukas Pesek |
Auto Kelly - CP | Aprilia | +1.224 |
| 12 |
Raffaele De Rosa |
Scot Racing Team | Honda | +1.456 |
| 13 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +1.487 |
| 14 |
Karel Abraham |
Cardion AB Motoracing | Aprilia | +1.706 |
| 15 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +1.790 |
| 16 |
Roberto Locatelli |
Metis Gilera | Gilera | +1.844 |
| 17 |
Shoya Tomizawa |
CIP Moto | Honda | +2.724 |
| 18 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +2.829 |
| 19 |
Imre Toth |
Team Toth Aprilia | Aprilia | +2.989 |
| 20 |
Aitor Rodríguez |
Milar - Juegos Lucky | Aprilia | +4.041 |
| 21 |
Axel Pons |
Pepe World Team | Aprilia | +4.153 |
| 22 |
Bastien Chesaux |
Racing Team Germany | Honda | +4.795 |
| 23 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +5.216 |
| 24 |
Valentin Debise |
CIP Moto | Honda | +5.357 |
| 25 |
Iván Maestro |
Milar - Juegos Lucky | Aprilia | +6.054 |
| 26 | 
Toby Markham |
C&L Racing | Honda | +8.530 |