Calificación 250cc - Gran Premio de Holanda 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Héctor Barberá |
Pepe World Team | Aprilia | 1:40.019 |
| 2 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.173 |
| 3 |
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | +0.191 |
| 4 | 
Marco Simoncelli |
Metis Gilera | Gilera | +0.301 |
| 5 |
Aleix Espargaró |
Balatonring Team | Aprilia | +0.777 |
| 6 |
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | +0.811 |
| 7 |
Raffaele De Rosa |
Scot Racing Team | Honda | +1.004 |
| 8 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +1.036 |
| 9 | 
Mike Di Meglio |
+1.053 | ||
| 10 | 
Lukas Pesek |
Auto Kelly - CP | Aprilia | +1.171 |
| 11 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +1.216 |
| 12 |
Roberto Locatelli |
Metis Gilera | Gilera | +1.328 |
| 13 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +1.443 |
| 14 |
Karel Abraham |
Cardion AB Motoracing | Aprilia | +1.514 |
| 15 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +1.526 |
| 16 |
Shoya Tomizawa |
CIP Moto | Honda | +2.019 |
| 17 |
Jules Cluzel |
Matteoni Racing | Aprilia | +2.609 |
| 18 | 
Stevie Bonsey |
Milar - Juegos Lucky | Aprilia | +2.678 |
| 19 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +3.056 |
| 20 |
Axel Pons |
Pepe World Team | Aprilia | +3.451 |
| 21 |
Valentin Debise |
CIP Moto | Honda | +3.634 |
| 22 | 
Imre Toth |
Team Toth Aprilia | Aprilia | +3.894 |
| 23 |
Bastien Chesaux |
Racing Team Germany | Honda | +4.685 |
| 24 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +4.724 |
| 25 | 
Toby Markham |
C&L Racing | Honda | +6.739 |