Calificación 250cc - Gran Premio de Indianápolis 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Mike Di Meglio |
1:44.341 | ||
| 2 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.120 |
| 3 | 
Marco Simoncelli |
Metis Gilera | Gilera | +0.212 |
| 4 | 
Héctor Barberá |
Pepe World Team | Aprilia | +0.284 |
| 5 |
Jules Cluzel |
Matteoni Racing | Aprilia | +0.519 |
| 6 |
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | +0.614 |
| 7 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +0.817 |
| 8 |
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | +0.851 |
| 9 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +1.055 |
| 10 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +1.097 |
| 11 | 
Lukas Pesek |
Auto Kelly - CP | Aprilia | +1.098 |
| 12 |
Roberto Locatelli |
Metis Gilera | Gilera | +1.219 |
| 13 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +1.437 |
| 14 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +1.836 |
| 15 |
Raffaele De Rosa |
Scot Racing Team | Honda | +1.854 |
| 16 |
Karel Abraham |
Cardion AB Motoracing | Aprilia | +2.567 |
| 17 |
Valentin Debise |
CIP Moto | Honda | +3.497 |
| 18 |
Axel Pons |
Pepe World Team | Aprilia | +4.117 |
| 19 | 
Imre Toth |
Team Toth Aprilia | Aprilia | +4.309 |
| 21 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +5.466 |
| 22 |
Bastien Chesaux |
Racing Team Germany | Honda | +5.547 |
| 23 | 
Adam Roberts |
Rat Racing | Yamaha | +6.955 |
| 24 | 
Barrett Long |
Longevity Racing | Yamaha | +7.813 |
| 25 |
Shoya Tomizawa |
CIP Moto | Honda | +0.000 |