Calificación 250cc - Gran Premio de Italia 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | 1:52.804 |
| 2 | 
Marco Simoncelli |
Metis Gilera | Gilera | +0.014 |
| 3 |
Héctor Barberá |
Pepe World Team | Aprilia | +0.038 |
| 4 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.246 |
| 5 |
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | +0.769 |
| 6 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +0.898 |
| 7 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +1.007 |
| 8 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +1.071 |
| 9 |
Raffaele De Rosa |
Scot Racing Team | Honda | +1.137 |
| 10 |
Roberto Locatelli |
Metis Gilera | Gilera | +1.184 |
| 11 | 
Karel Abraham |
Cardion AB Motoracing | Aprilia | +1.248 |
| 12 | 
Jules Cluzel |
Matteoni Racing | Aprilia | +1.407 |
| 13 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +1.547 |
| 14 |
Mike Di Meglio |
+1.559 | ||
| 15 |
Lukas Pesek |
Auto Kelly - CP | Aprilia | +1.571 |
| 16 |
Shoya Tomizawa |
CIP Moto | Honda | +2.188 |
| 17 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +2.657 |
| 18 | 
Imre Toth |
Team Toth Aprilia | Aprilia | +3.397 |
| 19 |
Axel Pons |
Pepe World Team | Aprilia | +4.770 |
| 20 |
Valentin Debise |
CIP Moto | Honda | +4.895 |
| 21 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +5.660 |
| 23 |
Daniel Arcas |
Milar - Juegos Lucky | Aprilia | +6.709 |
| 24 |
Bastien Chesaux |
Racing Team Germany | Honda | +6.813 |