Calificación 250cc - Gran Premio de Portugal 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Héctor Barberá |
Pepe World Team | Aprilia | 1:40.596 |
| 2 |
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | +0.058 |
| 3 | 
Marco Simoncelli |
Metis Gilera | Gilera | +0.088 |
| 4 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.283 |
| 5 |
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | +0.412 |
| 6 |
Raffaele De Rosa |
Scot Racing Team | Honda | +0.573 |
| 7 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +0.586 |
| 8 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +0.594 |
| 9 | 
Jules Cluzel |
Matteoni Racing | Aprilia | +0.609 |
| 10 |
Mike Di Meglio |
+0.757 | ||
| 11 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +0.941 |
| 12 | 
Karel Abraham |
Cardion AB Motoracing | Aprilia | +1.132 |
| 13 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +1.168 |
| 14 |
Roberto Locatelli |
Metis Gilera | Gilera | +1.198 |
| 15 |
Shuhei Aoyama |
Racing Team Germany | Honda | +1.598 |
| 16 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +1.716 |
| 17 |
Shoya Tomizawa |
CIP Moto | Honda | +1.752 |
| 18 |
Lukas Pesek |
Auto Kelly - CP | Aprilia | +2.063 |
| 19 |
Valentin Debise |
CIP Moto | Honda | +2.831 |
| 20 |
Axel Pons |
Pepe World Team | Aprilia | +2.849 |
| 22 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +3.987 |
| 23 |
Bastien Chesaux |
Racing Team Germany | Honda | +5.009 |
| 24 |
Christopher Moretti |
Matteoni Racing | Aprilia | +7.375 |
| 25 | 
Imre Toth |
Team Toth Aprilia | Aprilia | +8.199 |