Calificación 250cc - Gran Premio de Qatar 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | 2:00.677 |
| 2 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.100 |
| 3 | 
Mike Di Meglio |
+0.436 | ||
| 4 |
Héctor Barberá |
Pepe World Team | Aprilia | +0.497 |
| 5 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +0.959 |
| 6 | 
Gabor Talmacsi |
Balaton Racing Team | Aprilia | +1.016 |
| 7 | 
Mattia Pasini |
Team Toth Aprilia | Aprilia | +1.022 |
| 8 |
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | +1.036 |
| 9 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +1.061 |
| 10 |
Roberto Locatelli |
Metis Gilera | Gilera | +1.162 |
| 11 | 
Karel Abraham |
Cardion AB Motoracing | Aprilia | +1.195 |
| 12 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +1.385 |
| 13 |
Jules Cluzel |
Matteoni Racing | Aprilia | +1.436 |
| 14 |
Raffaele De Rosa |
Scot Racing Team | Honda | +1.731 |
| 15 |
Lukas Pesek |
Auto Kelly - CP | Aprilia | +1.890 |
| 16 |
Shoya Tomizawa |
CIP Moto | Honda | +2.913 |
| 17 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +2.915 |
| 18 |
Imre Toth |
Team Toth Aprilia | Aprilia | +4.218 |
| 19 |
Axel Pons |
Pepe World Team | Aprilia | +4.563 |
| 20 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +5.652 |
| 21 |
Bastien Chesaux |
Racing Team Germany | Honda | +6.419 |
| 22 |
Aitor Rodríguez |
Milar - Juegos Lucky | Aprilia | +7.024 |
| 23 |
Marco Simoncelli |
Metis Gilera | Gilera | +0.000 |