Calificación 250cc - Gran Premio de República Checa 2009
| Pos. | Piloto | Equipo | Moto | Tiempo |
|---|---|---|---|---|
| 1 | 
Marco Simoncelli |
Metis Gilera | Gilera | 2:01.611 |
| 2 | 
Hiroshi Aoyama |
Scot Racing Team | Honda | +0.350 |
| 3 | 
Héctor Barberá |
Pepe World Team | Aprilia | +0.453 |
| 4 |
Alex Debón |
Aeropuerto-Castello-Blusens | Aprilia | +0.775 |
| 5 | 
Mike Di Meglio |
+0.815 | ||
| 6 |
Roberto Locatelli |
Metis Gilera | Gilera | +0.932 |
| 7 |
Mattia Pasini |
Team Toth Aprilia | Aprilia | +0.950 |
| 8 |
Alvaro Bautista |
Mapfre Aspar Team | Aprilia | +1.137 |
| 9 |
Raffaele De Rosa |
Scot Racing Team | Honda | +1.157 |
| 10 | 
Karel Abraham |
Cardion AB Motoracing | Aprilia | +1.200 |
| 11 |
Héctor Faubel |
Thai Honda PTT SAG | Honda | +1.288 |
| 12 | 
Ratthapark Wilairot |
Thai Honda PTT SAG | Honda | +1.310 |
| 13 | 
Thomas Lüthi |
Emmi - Caffe Latte | Aprilia | +1.321 |
| 14 |
Jules Cluzel |
Matteoni Racing | Aprilia | +1.386 |
| 15 |
Lukas Pesek |
Auto Kelly - CP | Aprilia | +1.985 |
| 16 |
Shoya Tomizawa |
CIP Moto | Honda | +2.340 |
| 17 |
Alex Baldolini |
WTR San Marino Team | Aprilia | +2.489 |
| 19 |
Axel Pons |
Pepe World Team | Aprilia | +4.739 |
| 20 |
Valentin Debise |
CIP Moto | Honda | +4.960 |
| 21 |
Bastien Chesaux |
Racing Team Germany | Honda | +5.477 |
| 22 | 
Imre Toth |
Team Toth Aprilia | Aprilia | +5.519 |
| 23 | 
Vladimir Leonov |
Viessmann Kiefer Racing | Aprilia | +6.751 |
| 24 |
Aitor Rodríguez |
Milar - Juegos Lucky | Aprilia | +6.989 |
| 25 | 
Toby Markham |
C&L Racing | Honda | +0.000 |